Background: Prostate cancer is the second most common cancer in men and the fourth most common cancer overall. 1.4 million new cases of prostate cancer diagnosed in 2020. PSA and Gleason's score (GS) before treatment are important diagnostic and therapeutic indicators. According to studies, GS degrades tumors while PSA is not cancer-specific. The current study studies the relationship between Ki67, GS, and PSA, which is used to grade tumors and predict patient prognosis