Pasote (Dysphania ambrosioides L.) is a natural medicinal plant that is well known in Indonesia, especially the people of North Sulawesi. This plant is known to contain flavonoids, terpenes, sesquiterpenes, pigmol, xylosides, coumarin and essential oils. Pasote plants are also known to have biological activities such as antimicrobial, cytoxicity, antioxidants, larvacides, antidiabetic, antiparasitic, antiviral and molluscidal. In this study, the extraction of pasote leaves with the maceration method using water and acetone solvents was carried out in order to determine the best type of solvent that can produce pasote leaf extract with the highest antioxidant activity. Determination of antioxidant activity was carried out using DPPH on a UV Vis Spectrophotometer at a wavelength of 517 nm. The results showed that pasote leaf extract with acetone solvent had a percent inhibition (% IC) of 38.77% and pasote leaf extract with water as a solvent had an inhibition percentage of 46.96%. This shows that the best solvent that can extract antioxidant compounds in pasote leaves is water. The IC50 acetone extract pasote leaves value 9.7 µg/mL. The water extract of pasote leaves had the most effective antioxidant activity or inhibitory power with an IC50 value of 1.32 µg / mL. So the water extract of pasote leaves is more active than the acetone extract of pasote leaves. For further activities, including production in industry, it is better to use water extract only