Aims: Blood transfusion saves life but there is risk of Transfusion Transmission Infection (TTI), like Hepatitis B Virus (HBV), Hepatitis C Virus (HCV), Human Immunodeficiency Virus (HIV), malaria and syphilis. Aim of this study to find prevalence of HBV and HCV in blood centre. Settings and Design: This is an observational study at Blood centre of Tertiary care hospital from January 2018 to December 2020. Methods and Material: Donor Screened and selected according to National Aids Control Organisation (NACO), blood units were collected and processed. HBV and HCV reactive cases were diagnosed by enzyme-linked immunoassay (ELISA) analyser. HBV and HCV reactive donors further processed for Liver Function Test (LFT), Prothrombin Time (PT) and Complete Blood Count (CBC) were done. Statistical analysis used: Performed with SPSS software version 15. Chi-square proportion test has been used to compare the proportions of HBV seropositive between voluntary blood donor collection (VBD) and replacement blood donor (RBD) groups. Results: Blood units collected were 9,750,) numbers of VBD was ) 5,305 and RBD was 4425, during the study periods of three years in the blood centre, HBV and HCV reactive cases were diagnosed 50 and 02 respectively, and 35 HBV reactive in RBD. The p value of chi square test for testing two group (proportions of HBV infection between VBD and RBD group) proportions at 95% confidence interval is 0.0017. Conclusions: TTI can be prevented by promoting the voluntary donors, educate donors for TTI during donor screening, evaluate the methods of TTI screening.