Introduction: Urinary Tract Infection is the commonest infection in the community acquired and as well as hospital set up. Mechanisms of antimicrobial resistance in Enterococcus species are a significant concern. Objective: The aims and objective of our study was to know the prevalence of Enterococcus species as an urpathogens and fosfomycin resistance and the prevalence of FosB gene by sequencing in Enterococcus faecalis. Material and Method: We modified a PPF disk (Fosfomycin with sodium phosphonoformate) based on disk potentiation test which is expanded the inhibition zone around the PPF disc in the presence of FRGST producers. The production of (FR-GSTs) in the FREf strain using PPF disc was negative for FR-GSTs. Further fosfomycin resistant Enterococcus faecalis was confirmed by using PCR. Identified FREf were harbour with fosB gene. Result: In this study, Out of 618, 49(7.93%) specimens were significant with Enterococcus faecalis clinical isolates, 9 Enterococcus faecalis were resistant to fosfomycin (MIC ≥512 µg/l), 4 strains were intermediates to fosfomycin (MIC=128 µg/l) and 36 were susceptible to fosfomycin (MIC ≤64). The prevalence of 18.4% fosfomycin resistant Enterococcus faecalis (FREf) was found in our study. Conclusion: This finding prove that high expression level of fosB gene and Modification in the fosfomycin target enzyme MurA were the main reason of resistant mechanisms in these fosfomycin resistant Enterococcus faecalis. There are many studies done on the fosfomycin drug susceptibility and resistance mechanisms but still not completed. So, further, must urgently more research on fosfomycin resistant mechanism FosB gene