ABSTRACT Introduction: Many Gram negative bacteria are multi resistant as they produce ESBLs which breaks down the ring in the antibiotics there by rendering them ineffective. Infections caused by ESBL can be of great consequences even with common infections like urinary tract infections. Timely detection of drug resistant bacteria is of outmost importance as it plays a role in treatment approach and fatality. Materials and methods: 5ml of Clear midline stream urine was collected from 164 patients. The urine samples were careened for bacterial isolates and the presence of ESBL producers were confirmed by combination disc method and tested for antibiotic sensitivity and resistance. Results: A total of 164 urine samples from 82 males and females were collected. We found that 58 females and 32 males had recurrent UTI. 43 and 38 isolates of ESBL producing bacteria were found by combination disc method in urine samples of females and males respectively. Out of 81 isolates of ESBL producers 43 were from E coli and 20 were from K pneumoniae. Conclusion: We found that E coli is the largest producer of ESBL in urine samples of patients with diabetes. ESBL isolates were found more in female urine samples than in males. There was a strong positive association of levels of diabetes with ESBL production. The ESBL were highly sensitive to amaikacin and nitrofurantoin but resistant to 3rd generation cephalosporin.