High levels of depression, anxiety, and stress (DAS) can adversely influence the academic and clinical performance of nursing students, psychological well-being, and physical health. Large numbers of studies have recognized the factors that related by depression, anxiety, and stress. The purpose of the study is to determine the prevalence of depression, anxiety, and stress and their levels among undergraduate nursing students in the Mosul University /Mosul/ Iraq. A confidential questionnaire was done in this crosssectional survey study which is carried out using a depression anxiety stress scale DASS reported by Lovibond and Lovibond (1995a) carried out from 22th of January- 20th of March 2020. Among 800 nursing students analyzed, the age distribution shows that (83%) and (67%) respectively in the third class in morning and evening courses are between age group 22-25 years, 84% and 87% are female in the third class with significant p values. About the prevalence of moderate stress (26%) of third and 25% of second class respectively of morning and evening courses and mild anxiety 26% and 28% respectively of morning and evening courses. Finally, moderate depression was seen among 24% of the fourth class of morning and 35% of second class evening courses. The DAS and their levels among learner nursing students in the Mosul University, Mosul, Iraq had a high prevalence in comparison with others. Prospect research can localize on the need to set up social and mental health screening and hold up services, such as psychotherapy centers in nursing colleges.