Due to the less toxicity, cost effective and eco friendly nature plant derived nanoparticles has been increasingly gain the attention. In this study, prepared the silver nanoparticles using the extract of roots of Calotropis Procera medicinal plant used for the treatment of asthma. This method allowed the synthesis of silver nanoparticles, which was confirmed by FTIR, XRD and SEM. X-ray diffraction characterization of silver nanoparticles indicate that the structure of silver nanoparticles is face centred cubic structure of metallic silver. The topography and surface morphology of silver nanoparticles was examined by using Scanning Electron Microscopy (SEM) and the energy dispersive spectrum revealed that the presence of elemental silver in the sample. SEM images shows that most of the silver nanoparticles are predominantly spherical in shape with close compact arrangement with each other FTIR evidenced the presence of the functional group and where the reduction occurs and formation of silver nanoparticles which can be studied further to understand the chemical and molecular interaction which could be responsible for nanoparticle formation.