ABSTRACT: This study aims to develop a biology practical video (V-Lab) and a practical manual to assist teaching and learning of form four (students aged between 16 and 17 years old) biology subject. The study employed the development design and the ASSURE model was the main model referred. This study was conducted in a school laboratory involving teachers and students as respondents. Twelve practical experiments havebeen videotaped. Each video was edited by the inclusion of text, music and shortened to a period of 60 seconds. The practical manual was also prepared to assist the video. The V-Lab and practical manuals are validated by experts with a 92% of expert agreement and have a Cronbach Alpha reliability score of 0.82. The suitability of V-Lab and practical manual were also high (mean = 4.25, SD = 0.67) from the students' point of view. The conclusion is that V-Lab together with the practical manual are good and suitable for teaching and facilitating biology practical. The implication is that both V-Lab and the practical manual can be used to help, and guide teachers and students understand biology practical work step-by-step in theory before conducting real practical in the laboratory. Besides that, teachers and students can watch V-Lab any time and anywhere they like.