ABSTRACT: Under storage a challenge has happened population decreases drastically very next it gets its peak. This seems an opportunistic challenge to bring of such substrates where certain microorganisms might be replicated and stored for long time to be used by farmers. Many substrates from many researches came into record and has been used for mass production of antagonistic microorganisms (BCAs) known for biological control of other group of microorganisms called plant pathogens. Therefore, few tree borne oilseeds (TBOs) known for their medicinal and nutritional properties been used under current investigation. There were four cakes of Neem, Jatropha, Mahua and Karanja evaluated and found supporting population dynamics of Trichoderma harzianum when used as substrate. It was revealed that Neem cake was the best substrate in vitro supported Trichoderma growth for four months while Jatropha cake was found supporting population dynamics in vivo. The antagonist could be stored for more than 4 months when mixed with the sources of carbon and nitrogen. Among all substrates Neem cake found maintaining high population at any level when counted as colony forming units (CFUs) per gram of substrate.