The marine actinomycetes are a rich source for novel biomolecules. Especially the exotic tropical marine habitat of the Kerala coastal region favours the actinomycete diversity. The present study focuses on the isolation, purification and morphological characterization of marine actinomycetes. A total of 280 morphologically distinct actinomycetes were isolated from marine soil and sediments of 10 different isolation sites along the coastal region of Thiruvananthapuram district Kerala, India. The physicochemical analysis of the soil samples collected from different stations was also done. Even though the soil/sediment samples were collected from geographically nearby places, the physicochemical parameters showed a significant variation. This may be one of the factors which may trigger the actinomycete diversity in these regions