The current study aimed to show the Pharmacological effect of Panax ginseng against oxidative stress that induced by shigella in rats. 24 rats (wt 200-250 gm with age4-6 month) were used in current work and divide as follow; Control group: male rats received standard diet. Infected group: administrated with 1 x 109 S. flexneri. Root extract group: administrated with root extract 150mg/kg for month. Treated group: administrated with 1 x 109 S. flexneri and treated with root extract 150mg/kg for month.The results demonstrated significant (P < 0.05) increased in levels of malondialdehyde (MDA) with significant (P < 0.05) decreased in levels of glutathione (GSH) and catalase in an infected group compared with control group. The findings of Root extract group and treated group demonstrated non-significant (P < 0.05) difference in oxidative status compare with control group when using C. azarolus extract. It was concluded from present study that Panax ginseng extract has been role against S. flexneri.