Abstract: The energy supply of liver functions is carried out mainly by aerobic mitochondrial processes. We have studied some aspects of carbohydrate energy metabolism in mitochondria of the liver at the chronic influence of dichloroethane complex of factors, noise and vibration on experimental animals’ organism. The research aims to study some parameters of carbohydrate energy metabolism in mitochondria of the liver under the chronic influence of dichloroethane, noise and vibration. The researchers carried out experiments on 98 purebred white male rats weighing 160-180 g. Dichloroethane in the form of 10% oily solution was injected into the animal’s stomach in a dose of 1/20 LD50 (255 mg/kg), in conditions of noise (95-110dBa) and vibration (2-16dBa) during 15 and 90 days. After the experiments end in 15, 30, 60 and 90 days after exposure cessation to chemical and physical factors, the rats were decapitated and determined some indicators of carbohydrate and energy metabolism in the liver and blood of experimental animals. Presented results of experimental studies show that at repeated exposure to dichloroethane, noise and vibration there was observed an increase in the intensity of anaerobic glycolysis in blood, which is confirmed by the accumulation of lactic acid and reduction of glycogen. Similar changes were observed in the reduction of activity of alkaline phosphatase in the liver. Inhibition of enzyme activity (dehydrogenase) in all studied biospheres can be explained by the influence of dichloroethane, noise and vibration on enzyme functional activity. Inhibition of enzymes GDG, MDG, SDG and alkaline phosphatase in the liver is observed at multiple effects of dichloroethane, noise and vibration. As a result of the reduction of redox enzymes, under-oxidized products of anaerobic glycolysis of lactic, pyruvic acid are accumulated and the level of glycogen is reduced. Prolonged exposure to various chemical and physical factors in concentrations causing external effects in the experimental animals’ organism is manifested by hidden changes in several physiological reactions, biochemical indicators of the individual organs function and systems. Confirming influence of xenobiotics on cellular structures, mainly in the discoordination of functional activity of organs and specific enzyme systems.