Background & Aim: Chronic Otitis Media (COM) is a common curable cause for hearing impairment in developing countries. Eradication of disease from middle ear and repair of tympanic membrane perforation reduces the progression of hearing loss due to infection induced osteitis of ossicles. Tympanoplasty is usually done in quiescent stage of COM. Eustachian tube function, allergies and duration of disease affect the outcome of surgery. Our study aimed to assess middle ear mucosa for microbiological flora in COM Mucosal disease Inactive stage and its influence on outcome.