Background: one of the major causes of infertility is a defective sperm. The male factor of couple infertility is estimated to be about 25 percent to 50 percent. Sperm DNA integrity is important to successful fertilization and the normal development of embryos. Furthermore, sperm oxidative stress (OS) is associated with decreased sperm motility, decreased acrosome reaction, DNA damage and lower levels of implantation for in vitro fertilization. Materials and Methods: The study was conducted on two groups of 40 infertile men and 40 fertile men. Blood sample and semen sample was collected. Result:Seminal MDA was highly increased found in infertile men compared to fertile healthy men and serum level of antioxidant are low in infertile men as compare to fertile healthy men. Conclusion:The present study concludes that oxidative stress was involved in low sperm quality and male infertility's etiological agent, they should take the antioxidant rich diet and antioxidant therapy to boost infertility, and the measurement of MDA could be a useful diagnostic tool to assess oxidative stress.