Background: Patient rights may be considered as one of the main bases for defining the standards of hospital services. On the other hand, the concept of patient rights has been on the rise alongside the ever-growing interest of international organizations in human rights. In spite of the achievements concerning patient rights and ethical problems in healthcare, several problems still persist, including the unethical behavior from nurses, employees, and patient understanding of the concepts of the patient rights. Aim: The aim of the study was to assess nurses’ and employees’ compliance toward patients' rights and its relation to patients’ satisfaction. Setting: The study was carried out in Medical Surgical department at the Makkah Hospital. Research Design: A crosssectional survey design.Subject: participated of the study included a convenience of patients’ total number (n=100) patient admitted tomedical surgical department in hospital, and all staff nurses who working in department during the period of data collection. Their total numbers were (45) nurses and (10) employees. Tool: Two tools were used; Nurses’ and employees’ compliance to the patient’s rights questionnaire and patient satisfaction scale. Results: there was more than half of nurses have high level of compliance toward patients' rights as (50.9%) while, employees have high evel of compliance toward patients' rights as (49.9%), less than half of nurses have moderate level of compliance toward patients' rights as (48.1%) while, employees have compliance toward patients' rights as (48%) also none of nurses have low level of compliance toward patients' rights while, employees have very low compliance toward patients' rights as (2.1%) .The majority (87.3%) of patient exhibit the responses toward moderate level and (12.7%) of them responses toward low level of satisfaction. Recommendation: Nurse need to attend training program about patient rights that to increase patient satisfaction, also the hospital should made policy, rules and regulations that improve patient rights