Toxoplasma gondii is an obligate intracellular protozoan parasite causes toxoplasmosis, is one of the most prevalent human diseases in many countries [2, 19]. Reports on the prevalence of T. gondii infection among infertile females are limited, No previous reports about the relation between T. gondii infection and female infertility although about 21% of abnormal embryos and 24.2% of miscarriages and stillbirths have been related to T. gondii infection [21]. The present study aimed to survey the infertile females and Toxoplasma infection investigating a possible relationship with infertility. Sera from 18 Toxoplasma infected women and sera from 30 free Toxoplasma infertile women were tested by ELISA IgM and IgG toxoplasma antibodies, and the level of serum IL4, IL-8, IL10 and IL33 was tested using ELISA technique.