ABSTRACT Introduction: The Bicipital Groove (BG) lies below the anatomical neck of humerus, separating the greater and lesser tubercles. It lodges the tendon of Long Head of Biceps Brachii (LHBB) and ascending branch of the anterior circumflex humeral artery. The shoulder pain is frequently caused by biceps tendon pathologies, which are associated with variations in morphometry of the BG. Therefore, this study was conducted to determine morphometric features of BG in Gujarat region of India to provide additional information. Materials & Methods: The data was collected from 100 dry adult humeral bones of unknown sex (50 right and 50 left) available in anatomy department of Pramukhswami Medical College, Karamsad, Gujarat, India. The digital Vernier caliper was used to precisely measure the BG’s length, breadth, depth, medial wall length, and lateral wall length. The opening angle (OA) and medial wall angles (MWA) was measured by screen protractor software after capturing and transferring images of bones to the computer. Statistical analysis was done on the collected data. Result: The length, width and depth of BG were 73.21 ± 9.08 mm, 10.15 ± 1.01 mm and 4.24 ± 0.69 mm on right side and 71.94 ± 8.45 mm, 10.11 ± 0.89 mm, 4.31 ± 0.83 mm on left sides respectively. The average opening angle on the right side was 76.11±13.79º, while on the left side was 76.11 ± 13.79º. The medial wall angle was 55.74 ± 11.92º on right side and 54.03 ± 8.94º on left side. Conclusion: This study provides additional information on the morphometry of the BG in the Gujarat region, which can be useful for anatomists, anthropologists, orthopaedic surgeons and radiologists. The results of this study may also be used to determine prosthetic design, size, and position in humeral head replacement.