Abstract: The present study included the collection of 121 samples from midstream urine for investigating the presence of K.pneumoniae in urinary tract infections, the samples were collected from Al-Shaheed Mohammed Baqir AL-Hakeem hospital and private laboratories in Baghdad province. The study was carried outthrough March 2019 to the beginning of June 2019. The samples were identified according the morphological and microscopically characteristics of the colonies after culturing on number of culture media as well as biochemical tests, molecular identification were also used as a final confirmed diagnostic test for isolates that were diagnosed as K.pneumoniae bacteria based on the blaTEM, blaSHV and blaCTX-Mgenes which has specific sequences for K.pneumoniae bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance. The results of the final diagnosis showed that 38 isolates belong to target bacteria, The examination of the sensitivity of all bacterial isolates was done for selected these isolates towards 16 antibiotics by aVitek2 compact ASTN system and the isolates were resistant for a number of antibiotics used such as; Amikacin (5.26%), Imepenem (5.26%), Ertapenem (7.89%), Meropenem (10.52%), Gentamicin (21.05%), Ciprofloxacin (26.32%), Cefoxitin (39.47%), Trimethoprim/Sulfamethoxazole (50%), Ceftriaxone (52.63%), Fosfomycin (55.26%), Piperacillin/Tazobactam (57.89%), Nitrofurantoin (57.89%), Ceftazidime (65.79%), Cefuroxime (71.05%), Cefixime (73.68%) and Ampicillin (100%). The presence of Extended Spectrum Beta-Lactamase genes in 38 K.pneumoniae isolates were 65.8 % of the ESBL genotypes expressed blaSHV genes followed by 52.6 % blaTEM and 42.1 % for blaCTX-M.