ABSTRACT: P.aeruginosa is a common cause of hospital-acquired infections, and it's especially troublesome in intensive care units. Pseudomonas aeruginosa shows intrinsic resistance to a number of antibiotics. Treatment of infection has become a challenge as number of antibiotic resistant strains is on the rise. In general, Pseudomonas aeruginosa resist antibiotic attack by intrinsic, adaptive, and acquired resistance mechanisms. Resistance can be acquired by transfer of genes coding resistance carried on plasmids, transposons, integrons or prophages or through mutational changes. Now, worldwide emergence of colistin resistance has also occurred. Colistin resistance in Pseudomonas aeruginosa can be caused by chromosomal mutations as well as by the acquisition of plasmid-carrying determinants, primarily mcr-1. Hence, this current study was aimed to detect the presence of mcr genes among MDR P.aeruginosa isolated from various clinical specimens.