ABSTRACT: Nematodes (Roundworms) are also known as strongylids parasites belonging to the order Strongylida, superfamily Trichostrongyloidea. Haemonchus contortus and Trichuris ovis species are most important nematode parasites, which are found in the gastrointestinal tract in the goat (Capra hircus). Molecular techniques were used to the identification of parasites and amplification is done with the help of ITS2 (Internal Transcribed Spacer 2) fragment of the ribosomal DNA. The present study revealed that the amplified the product band size of gastrointestinal nematode parasites were Haemonchus (325bp-MW595998.1), and Trichuris species (205bp-MW667589.1). The ITS2 sequencing and phylogenetic tree (Neighbour joining method by MEGA7.0 software) revealed 99 and 100% (Haemonchus contortus and Trichuris ovis) homology.