Abstract: The study included identification of Leishmania parasite from cases of cutaneous leishmaniasis, by examination of smears from patients and Nested-PCR technique ,Out of 215 cases suspected for cutaneous leishmaniasis included (61) pus and (178) tissue these pus sample were diagnosed by direct smear method to see the Amastgote phase .The cases included in this study belonged to some hospitals in the Babylon as Morjan Medical City Hospital (24) , Musayyib General Hospital(17), Thu AlKifl General Hospital (29) and Hashimyah General Hospital(145), during the period from (October 2019 to March 2020). According to stain results,(60.6%) from the (61) total pus sample cases were positive to giemsa stain and after that used the DNA extraction from total number (215) sample in Nested–PCR and the results appear in percent (82.7%) ,these percent divided in to (20.7%) to Leishmania. Tropica and (79.2%) to Leishmania. Major. The study aimed to detection of Leishmania species in Babylon province.