Background: Hospital-associated infections are the major cause of patient morbidity and mortality. Environmental monitoring by the microbiological testing of surfaces and equipments is useful to detect changing trends of types and counts of microbial flora. Aim: Identify bacterial colonization of surface and equipments isolated from Operation theatres, ICUs and NICUs of a tertiary care hospital in Jammu region. Methods: Swabs were taken from different sites and equipments and bacterial species were isolated and identified from them. Results: Among contaminants, Micrococci (30%) and Bacillus spp. (25%) were the most common. The bacterial pathogens isolated were Klebsiella spp. (14%), Coagulase Negative Staphylococcus aureus (11%), Pseudomonas sp. (2%), Acinetobacter spp. (11%), Staphylococcus aureus (5%), and Enterococcus spp. (2%). Conclusions: High level of microbial contamination indicates the needs for periodic surveillance aimed at early detection of bacterial contamination levels and prevention of hospital acquired infections.