Background :Gloves are used during all patient-care activities that involve exposure to blood and all other body fluid, during contact precautions and outbreak situations.. The non sterile gloves are appropriate for examination and non surgical procedures and sterile gloves for surgical procedures. In developing countries like India where the money set aside for public health is less than 1% of the gross domestic product (GDP), routine use of disposable materials like gloves for examining patients as well as for minor surgical procedures can be a drain on the resources. The use of surgical gloves when not indicated represents a waste of resources and increases the treatment cost. Recently there have been studies indicating use of clean examination gloves while performing minor oral surgical procedures. So we decided to conduct study to ascertain microbiological quality of surgical and clean examination gloves in oral surgery to determine if there was a significant difference in bacterial colony forming count (CFUs) and evidence of post operative infection between two types of the gloves. Material and method: A randomised comparative double-blind study was conducted on 100 patients requiring minor oral surgical procedures like extractions of multiple teeth using sterile or clean examination gloves. The microbiological specimens were collected from gloves by saline dilution method and incubated for 7 days on trypticase soy agar.The number of colonies were counted and expressed as total colony forming units. The patients in both groups were examined for assessment of post operative complications like infection to compare the co relation between no of CFU s and infection rate. Statistical analysis:All results were tabulated and statistically analyzed using SPSS version 20. Results: The intergroup comparison of the pre- and postoperative bacterial counts (CFUs) with the t-test paired showed highly significant 1 percent significance difference (P < 0.01) . The patients in both groups were examined for assessment of post operative complications like infection to compare the co relation between no of CFU s and infection rate and was found that none of the patients were found to be infected at 7th day recall in both groups. . Conclusion:It was concluded that minor oral surgical procedures can be carried out with clean examination gloves, without any increased the rate of infection