Background: Patients admitted to intensive care units have been found to experience HAIs at a rate 5-10 times higher than patients treated to normal wards. The present study was conducted to find microbial strain pattern in blood and urine and their sensitivity to drugs with patients on mechanical ventilation. Materials & Methods: 100 patients between the age of 18 to 65 years on mechanical ventilation of both genders in the department of Medicine, Gandhi Medical College and associated Hamidia Hospital, Bhopal were recruited. Parameters such as TLCs, neutrophil counts and lymphocyte counts were recorded. Micro-organism in Blood and urine was calculated. Antibiotic susceptibility pattern was also recorded.