ABSTRACT Nosocomial infection hospital has always acted as source of infection to patient admitted to them. The infection usually occurs after the patient admitted to hospital. The incidence of hospital infection has been reported to be 2 to 12 percent in advanced countries but it is much higher in crowded hospitals. Such infection is evident during their stay in hospitals or only after discharge [7]. 100 samples were taken from the Catheterized patients for the study from which 45% E.coli and 22% Klebsiella spp. and 19 Pseudomonas spp. were isolated. The pattern of antibiotic susceptibility suggested that 100% of the isolates were resistant to Norfloxacin and 27.2% Amikacin, 18.1% Ciprofloxacin, Imipenem, Meropenem and the least resistance was shown by Gentamicin, TobramycinPiperacillin 9.0%. Antibiotic resistance pattern shows that all of the 22 isolates were Multidrug resistance Klebsiella (MDR)