INTRODUCTION:Thyroid hormones are essential throughout life for the regulation of development, calorigenesis, and metabolic rate. They operate on almost all organs in the body, including the gut and the visceral, so disturbances in the function of the thyroid may have gastrointestinal manifestations and concomitant effects on the immune system on body tissues. It is possible that cytokines play an important role in thyroid autoimmune disease. IL-6 is a 26 KD protein that is produced by fibroblasts, endothelial cells, monocytes, and T lymphocytes. Typically, hyperthyroidism can cause limited serum calcium elevation. In up to 20 percent of patients with hyperthyroidism, asymptomatic serum calcium elevation has been reported and is due to increased bone resorption and subsequent release of calcium from the bone into the bloodstream.There are well-known effects of thyroid hormones on mitochondrial oxygen intake. Total antioxidant status (TAS) provides information on all antioxidants in the organism, while Malondialdehyde (MDA) is a marker of lipid peroxidation that is used to measure lipid peroxidation due to increased oxidative stress. METHODS:The study was carried out at SMHRC, Maharashtra India during the period of August 2019 to May 2020. We enrolled 150patients (50 hyperthyroidism Female, 50 hypothyroidism Female and 50 normal females) aged 25-50 years. The control subjects and patient groups were not taking any medication that affect thyroid metabolism.Serum IL-6 was measured by an enzyme-linked immunosorbent assay.MDA was estimated by TBA method. Results: In hyperthyroid women's cells, the development of IL-6 by blood mononuclear cells was higher. Substantial increased levels of IL-6 were observed in untreated hyperthyroid patients relative to healthy controls (p<0.001). Hyperthyroidism may be associated with hypercalcemia symptoms. Compared to controls, MDA was elevated in hypothyroid patients. Conclusion: We found that serum IL-6 increased in hyperthyroidism patients, but reduced in hypothyroidism patients, and normal in control group. The correlation of hypercalcemia with hyperthyroidism should be known to the clinician. These findings indicate an increased malondialdehyde in hypothyroid states, which can be clarified by both the inadequate rise in antioxidant level and the altered lipid metabolism in these cases. This may promote early diagnosis and effective intervention.