Background: The present study was conducted to assess knowledge, awareness and practice of dental practitioners regarding COVID-19 pandemic. Materials & Methods: 240 dental practitioners of both genders were provided with a questionnaire regarding knowledge and practice regarding COVID-19 pandemic. Results: 80% showed that SARS-CoV-2 is the cause of COVID- 19. 85% replied that 2-14 days is the incubation period of Covid- 19, 75% correctly replied that 6.8 % is the incubation period for COVID- 19 and 82% replied that rRT-PCR is the laboratory test available for detection of COVID- 19. 80% replied yes in order to provide treatment to infected patients and 10% replied no. In response to question whether masks, head cap and sanitizer protect against virus, 74% replied yes, 18% replied no and 8% replied don’t know. The difference was significant (P< 0.05). Conclusion: Dental practitioners had sufficient knowledge, awareness and practice regarding COVID-19 pandemic.