ABSTRACT This study aimed to isolate, purified and identified staphylococcal enterotoxin A from Staphylococcus aureus. 200 clinical urine samples were collected from pregnant women with urinary tract infection aged from ( 20-40 years).Then, these isolated were identified as Staphylococcus aureus by biochemical and molecular methods. The percentage of positive culture of Staphylococcus aureus was 47%. One strain was selected to produce of Staphylococcal enterotoxin A and this enterotoxin was purified by chromatography technique such as ion exchange and gel filtration. All purified fractions were collected and measured at 280 nm and then presence of enterotoxin A was detected by ELISA kit. Finally ,50 mg\ml of SEA was extracted from 15 liters of brain heart infusion broth.