Abstract: Cesarean section is the most frequent surgical procedure in obstetrics. This study was carried out to isolating and identifying bacteria causing cesarean section inflammation from patients and antimicrobial susceptibility test to bacterial isolates; 30 samples were collected from patients with different aged of women that had suffering from Post-Cesarean section Infection in Bent-Al-Huda & Al-Habboubi hospital in in Thi-Qar province. The isolates were identified on the bases of their morphological characteristics, the Gram stain reaction, biochemical tests and confirmed by the Api 20 E test. The results revealed that the rate of bacterial isolates in patients with Post-Cesarean section Infection was (76.6%). out of these (34.8 %) were E .coli, (30.4%) were Pseudomonas aeroginosa, (13%) of Staphylococcus aurous, (8.6%) isolates of Acintobacter and Bacillus. Antimicrobial susceptibility testing to 23 bacterial isolates showed variable sensitivity to antibiotic, they were more sensitive to chloramphenicol and Imipenem also the results appeared increasing prevalence of multidrug resistant between isolates.