Background:- Mastitis is the term for a bacterial infection of the udder. It is most common in ewes raising multiple lambs or with high milk production. Most cases occur during the first weeks after lambing or immediately before weaning. Good ewe nutrition and providing a clean lambing environment are important factors in reducing the incidence of mastitis. Objective: The study was focused on using of rapid & efficient methods for detection of Subclinical Mastitis in 4 small holder dairy flock in central of Kirkuk &its affiliated areas. Material and Methods:- A total of 94 milk samples sheep were collected from four farms in October to December 2018 to determine pathogens responsible for subclinical mastitis in sheep. Results:- Screening Subclinical Mastitis(of SCM) were done using California mastitis and White Slide Test (WST). the prevalence percentage of SCM in California mastitis and White Slide Test according the early stage lactation was(74 % and 70%) respectively. While the prevalence percentage of SCM in California mastitis and White Slide test according the according the dry period lactation was(66 % and 64 % ) respectively. identification of the isolates was achieved using Gram’s staining, hemolytic pattern, colony morphology, Catalase , Coagulase test, IMVIC test and confirmation of bacteria species by using Vitek 2 compact after identification to the primary biochemical tests using Analytical Profile Index. Bacteriological examination of all milk samples found the presence of (82.85% ) isolates where Staphylococcus was predominant species (74.28 %) and the coagulase negative Staphylococcus (25.7% ) species was identified at the least bacteria .than staph. aureus, while environmental pathogen represented E. coli second common pathogen followed by klebsiella pneumonia (14.8% ) , Manhemia haemolytica (12.8 %) proteus spp.( 11.4%) And low percentage to the Enterobacter spp. (4.3 %) . Conclusion:- subclinical mastitis seems to be, as deduced from the high prevalence observed in this study, an important health problem for milking sheeps in the Kirkuk Application of VITEK system could be practiced at selected samples from time to time to confirm identification of causative organisms.