Mangroves is one of the chief ecosystem which thrives in tropical and subtropical intertidal zones. It plays an important role in safeguarding beaches and rivers from prefunding sea waves, air pollutants and storms. Since these plants can withstand high biotic and abiotic stress, they can be a source of novel biotechnologically useful products. Mangroves are considered as the most productive ecosystem harboring a variety of flora and fauna. It is a home to a huge number of unique fungal communities known as manglicolous fungi. In the current study mangroves of unexplored location of Kutch, Gujarat, India was investigated for the presence of endophytic fungi. Total 13 different fungi were isolated from various root samples. The fungi were identified based on morphological characteristics. Where, Penicillium sp, Aspergillus sp. and Alternaria sp. was the most commonly found genera.