The breasts are the protrusions in the upper abdominal area of the male and female primate trunks. In women, the breasts act as mammary glands to produce and store milk for babies. Certain parts of the breast can become cancerous, like other tissues and organs. Breast cancer is very common. One of the parts of the breast where tumors may form is the lobules. These tumors are also called carcinomas. There are many breast cancer treatments, including surgery, radiation therapy, hormone therapy, and chemotherapy, each with its own side effects and disadvantages. This study investigates the effect of breast cancer cell migration after NGO exposure and also examines the expression and modification of T1MP1 and CYP1B1 proteins. Initially, MDA cancer cells were prepared and cultured. These cells were then treated by the NGO and examined by APOP ICC, SCRATCH, DAPPY, SRB tests. Studies have shown that in all of these tests, cell survival was reduced due to the NGO's effect on cancer cells. Cell survival was increased only in the SRB test by increasing graphene concentration, which can be attributed to NGO light absorption.