ABSTRACT To facing the growing need for new drugs, the investigation of medicinal plant continuing. So that in the current study, the antibacterial and antifungal and anticancer effect of H. sabdariffa water extract were studied. The antibacterial test done against Escherichia coli, streptococcus mutans and Pseudomonas aeruginosa were it gave the best inhibition zone 35 mm against E. coli. Furthermore the antifungal activity tested against Rhizoctonia solani, Fusarium graminearum and the best inhibition growth activity found against R. solani by 69%. The anticancer activity tested by MTT test against SR (lymphoma) and MCF7 (breast cancer) cell lines, the best cytotoxicity found against MCF7 where it reach 64%. In conclusion, the H. sabdariffa water extract have a promising properties in the discovery new drugs due to its biological activity and its viability worldwide.