Background: The study investigates the effectiveness of infection control protocols against the COVID variant JN1 in dental practices. With the evolving landscape of COVID variants, understanding the adequacy of current protocols is critical for ensuring the safety of both patients and healthcare practitioners. Methods: A mixed-methods approach was employed, encompassing direct observation, interviews, and environmental sampling across 20 diverse dental practices. The assessment criteria included PPE usage, environmental sanitation, patient triage, and staff training. Data were analyzed quantitatively and qualitatively to provide a comprehensive evaluation of infection control measures. Results: Adherence to PPE protocols was generally high, although variability was observed in face shield and gown usage. Environmental sampling revealed the presence of variant JN1 in sampled areas. Staff training rates were high, but disparities in protocol awareness and consistent implementation were noted. Correlation analysis demonstrated a negative association between adherence and viral presence, emphasizing the importance of strict compliance. Discussion: The findings underscore the significance of stringent adherence to infection control protocols in mitigating the transmission of variant JN1 in dental practices. Addressing variability in protocol implementation and reinforcing specific aspects of protocols are crucial for enhancing safety. Conclusion: This study provides valuable insights into the efficacy of infection control protocols against variant JN1 in dental practices, emphasizing the need for targeted interventions to strengthen protocol adherence and minimize the risk of viral transmission.