Abstract INTRODUCTION- Doctor’s stress has an impact for health and doctor’s satisfactory level involved which ultimately has an effect on care quality for the patients. Stress induced sleep disturbance is prominent. AIM-This study was designed with aim of assessing the influence of work characteristics on the association between doctor’s stress and sleep quality METHOD- This cross-sectional study was conducted on doctors of Tertiary Care Hospitals of Wardha city using questionnaire, containing 24 questions. The stress was evaluated using 10 items “Global perceived stress scale” and for sleep quality “Pittsburgh Sleep Quality Index” of total 14 items was used. The variables were compared in unpaired t test and Pearson correlation test to know the level of significant of determinacy which was set at less than 0.05. RESULTS –The Positive PSS Score for post-graduates was 14.03 ± 4.55 and staff was 15.69 ± 4.44, the Negative PSS score was 13.57 ± 5.15 and 11.37 ± 4.39, respectively which was statistically significant (p<0.05). Negative PSS scores of females were higher (13.92 ± 5.36) as compared to males (11.96 ± 4.54) but the difference was also statistically insignificant (p <0.05). The total PSS and Total PSQI scores were almost equals in post-graduates and staff, males and females, day and night shift duty doctors. CONCLUSION- Certain characteristic of work shift among doctors can directly affects the stress and sleep quality and indirectly affect the patient care and treatment. The study indicates that work characteristic may influence sleep quality and stress among doctors. We need to improve work shift schedule in order to provide good health and well-being of doctor which will further improve the quality of patient care.