ABSTRACT The fight against surgical infections remains an urgent problem in surgery. The problem of microflora resistance makes it difficult to obtain positive results of purulent-surgical diseases of soft tissues. In the literature, there are few studies devoted to the suppression of the existing microflora resistance to antibiotics. The aim of the study: There was a laboratory study of the microbiological activity of dimethylsulfoxide, the identification of the optimal concentration of the solution with the maximum bactericidal and suppressive properties of the resistance of pathogenic microflora in vitro. The results of the experimental in vitro studies have shown that 25% dimethylsulfoxide is the optimal concentration in terms of suppressing microflora resistance.