Abstract Background: A common metabolic issue in new born is neonatal hypoglycemia, which is more prevalent in conditions including preterm, sepsis, and for small gestational age. New born without obvious risk factors may experience episodes of asymptomatic hypoglycemia. Material and Methods: In a tertiary care facility, a hospital-based prospective study was carried out for a period of six months from August 2022 to January 2023. In the study, 104 new-borns who had successive deliveries were taken into account. At 3 hours, 12 hours, and 36 hours after birth, their babies have tested for plasma glucose levels. Using the SPSS (version 21) statistical program, the plasma glucose values were analysed statistically using descriptive and inferential statistics. Results: The incidence of asymptomatic hypoglycemia among new-borns weighing more than two kgs was 18.3%. The risk factors such as male gender, Low birth weight babies, caesarean delivery small for gestational age was significantly associated with asymptomatic hypoglycaemia. Blood glucose levels was significantly lower in SGA babies and caesarean birth babies. Conclusion: In neonates, asymptomatic hypoglycemia occurs quite frequently. In order to recognise asymptomatic hypoglycemia and implement preventative interventions to stop the transition to symptomatic hypoglycemia and its consequences later in life, strict monitoring and close attention are required.