The indoor air quality of public utility places is very important for human health. Bioaerosols concentration in ambient air quality conditions have effects on the ecosystem and human health. In this study, it is aimed to determine the diversity and number of indoor bioaerosols before and after using the nano-plasma air purification system in a room used as a clean room in Van Regional Training and Research Hospital and to determine its effectiveness. The air samples were collected using passive air sampling method 3 different places 4 Petri dishes (blood agar, EMB agar, SD agar, and chocolate agar, RTA laboratories, Turkey) for a while of 1 hour, 2 hours, and 4 hours in 75 m3 room. The collected agar plates were incubated in the microbiology laboratory of our hospital for 24 hours. Growing microorganisms were evaluated with a conventional and automated device (VITEK 2, bioMe´rieux, France). Before using the nano-plasma air purifier device, the culture results of bioaerosols were found 120 CFU / Petri and 18 different types of microorganisms and 4 hours after using the nano-plasma air purifier device, 20 CFU / Petri and 8 different types of microorganisms (Table 2,3 Figure 1,2). The most produced microorganisms are gram-positive bacteria while the air purifier nano-plasma device is used and the most dominant is Micrococcus spp. was determined. As a result, it was determined that air cleaning devices were very effective in decreasing the number and variety of microorganisms in the indoor air as a cleanroom in the hospital.