A novel periodontal film for the treatment of periodontitis was developed by using herbal drugs Quercetin and Rutin, it is a plant flavanol from the flavonoid group of polyphenols. It is found in many fruits, vegetables, leaves, seeds and grains; capers and red onions. It has a bitter flavour and is used as an antimicrobial agent and effective against infecting microorganisms in the periodontal pocket. Periodontal films were prepared by solvent casting technique using Eutragit and HPMC as polymers Dibutyl phthalate as plasticizers and PEG as surface active agent. The patches were cut in 1 cm 2 and placed in desiccator for further study . In vivo animal study was performed by using Male Albino rabbit (New Zealand rabbit) 8–12 weeks. The operative area was cleaned and periodontal patch of 1cm 2 was placed in the gingival sulculus of rabbit’s lower incisors. The gingival crevicular fluid of the lower incisors was then extracted using #30 standardized sterile paper point on 1, 2, 4, 7, 10, and 14hrs.