Abstract: Gender discrimination in today's organizations is one of the issues that if it cannot be managed well, there will be other problems in the organization that may cause great harm to the organization, Therefore, the object of this research is to examine the effect on workers of gender discrimination. This survey's statistical population is Ghalib Private University in Herat that 109 employees have been selected as a sample. The measurement tool of this research is a questionnaire that reliability is above 0.7 and has been confirmed. SPSS 24Software was used to study descriptive statistics and Smart PLS 3.0 software was used to analyze statistical data in inferential statistics. According to the results, gender discrimination has had a detrimental and critical impact on work satisfaction, employee participation, and organizational identity and has had a positive and significant effect on job stress.