Abstract Hepatitis infection burdens have been increasing and alarming around the globe in recent years. The mainstream therapeutics of hepatitis infections are non-specific with numerous side effects. To combat viral hepatitis, a novel therapeutic platform is needed. In the present study, we explored a novel biogenic approach of nanotechnology as an alternative therapeutic platform for treating hepatitis. Biogenic silver nanoparticles (PTNPs) synthesised from Pterocarpus santalinus bark extract were used in the treatment against induced hepatitis. DGalactosamine was given to male Wistar strain rats to induce hepatitis similar to viral hepatitis inflammations. The hepatic serum markers, Alanine aminotransferase and Aspartate aminotransferase were examined during the hepatitis conditions. The results obtained indicated that the Alanine aminotransferase and Aspartate aminotransferase enzyme levels were significantly reduced after the treatment with PTNPs. The hepatoprotective activity demonstrated from PTNPs would find application in alternate therapeutics in the treatment of hepatitis