Semarang has been reported as an endemic area of dengue hemorrhagic fever (DHF), yet continuous data of molecular epidemiology related to dengue fever is not available. The aim of this study was to analyze the characteristics of virus strains, host suspected dengue, and the dynamics of dengue fever in Semarang City. The design of this study was observational description with cross-sectional design. Seventy three DHF suspected patients from two hospitals and seven health centers in Semarang City were sampled during 2017- 2018. Reverse Transcription Polymerase Chain Reaction (RT-PCR) method was used to examine serum suspected dengue and sequence serotypes and genotypes of dengue virus. The results showed that the highest frequency to be exposed by DHF was infant and children; while, the number of male and female with suspected dengue was relatively the same. The dominant serotype was DENV-3, DENV-1, and mix of DENV-2 and DENV-3; while, the circulation of DENV-1 genotype I and DENV-3 genotype I were identified as Indonesian local endemic strain causing epidemics in Indonesia.