ABSTRACT DNA has become a powerful forensic tool for solving cases such as linking a suspect to a crime scene, resolving biological relationship issues and identifying disaster victims. DNA fingerprinting can be used to help identify individuals by revealing the differences in the DNA sequence among different people. Certain non-coding regions of DNA exhibit significant differences in base pair sequence among individuals. Such regions are called polymorphisms. DNA investigations mainly involve two steps; the obtaining of DNA profiles from biological samples and the interpretation of the evidence given by these DNA profiles. Chromosomal DNA was extracted from semen samples as well as from blood stain more over PCR technique applied to amplify STR loci, while the detection carried out by capillary electrophoresis .Then making the interpretation by matching DNA profiles that obtain from both suspects and victims with the DNA profiles that obtain from the objects in the crime scene. The case involve seven forensic samples, one female victim (A), three suspects (B, C, D) and three clothes (brown underwear, blue jeans and shirt belong to victim A).. DNA extraction from the semen contamination in these samples give DNA fingerprints with a mix profile that’s belong to more than one suspect.