Annotation: This article presents the results of an experimental cholestasis conducted on 69 white outbred male rats of a mixed population with an initial weight of 180-200 g. In 37 rats, extrahepatic cholestasis was reproduced by ligation of the common bile duct. The overall mortality rate in this group was 30.3%. False-operated animals (24 rats) served only as laparatomy under aseptic conditions. In these groups, mortality was not observed. The intact group consisted of 8 rats. Studies were carried out 1, 3, 7 and 15 days after the reproduction of models. The timing of the study was associated with the development of significant morphological and functional changes in the liver during experimental cholestasis. The activity of aminotransferases, alkaline phosphatase, cholesterol, bilirubin was determined in blood serum. According to the results of the experiment, it was revealed that cholestasis causes hyperfermentemia, hyperbilirubinemia, hypercholesterolemia. Extrahepatic cholestasis changes the activity of AOD enzymes in different directions, more pronounced changes in the activity of SOD were noted in the early stages. It is inhibited in the homogenate of the digestive system, amplified by the kidneys in the early days of the study and inhibited after 7 days. Catalase activity in homogenates of the liver, kidneys, pancreas and small intestine showed less variability.