Background: Chronic kidney disease (CKD) is an emerging public health problem that affects 5–10% of the world population. With the availability of recent treatment modalities including renal replacement therapy, the survival of CKD patients has increased considerably which has led to an increased focus on health-related quality of life (HRQoL). Health-related quality of life represents the impact of the disease or its treatment on the subjective feelings of patients about their physical, mental, spiritual, emotional, social, and functional wellbeing. Quality of life among dialysis patients has been shown to be lower as compared to pre-dialysis CKD patients and is an independent risk factor for mortality in dialysis patients. Family support is an important factor that serves as a support system for the patients to face health problems. Purpose: To examine levels of family support and the correlation of family support and quality of life (QoL) among CKD on hemodialysis (HD) patients. Methods: This cross-sectional observational study was conducted in August-November 2020 and recruited chronic HD patients from Wahidin Sudirohusodo Hospital in Makassar, Indonesia. The demographic data of HD patients were collected. Quality of life was assessed using the validated Indonesian version of the Short Form-36 (SF-36) questionnaire which consists of Physical Component Summary (PCS) and Mental Component Summary (MCS) and family support that was assessed using the validated “Dukungan Keluarga” questionnaire. The data were analyzed using SPSS version 22.0 statistical software. Results: A total of 60 subjects (39 males and 21 females) were included. The mean age was 48.9±12.1 years old. About 55% of the subjects was graduated from college. About 86.7% subject was married. About 55% of the subject is not working. About 53.3% of subjects have a family income lower than the regional minimum wage. The mean duration of HD was 22.1±28.6 months. The mean score of family support was 53±8,2. Family support score has a significant positive correlation with each domain of PCS and MCS, the higher score of family support is in accordance with higher score of PCS and MCS. Conclusion: CKD on HD patients’ quality of life was significantly affected by family support.