Background: The emergence of novel COVID-19 variants presents challenges across healthcare sectors, including dental practice. Understanding the knowledge and awareness levels of the JN1 variant among dental professionals is crucial for effective infection control. Objective: This study aims to assess the knowledge and awareness of the JN1 variant among dental practitioners and identify areas for targeted interventions. Methods: A cross-sectional survey was conducted among [n=300] dental professionals, evaluating their knowledge and awareness levels using a structured questionnaire. Descriptive statistics and subgroup analyses were employed to interpret the findings. Results: The survey revealed commendable understanding of the JN1 variant's transmission and preventive measures among respondents. However, awareness regarding recent scientific literature and diagnostic methods was relatively lower. Variations in knowledge and awareness were observed based on demographic parameters. Conclusion: While dental professionals exhibit promising knowledge in certain aspects of the JN1 variant, addressing gaps in staying updated with recent advancements is essential. Tailored educational interventions are necessary to fortify infection control measures within dental practice communities.