Abstract The results of using the algae alone and nano-algae extracts and Streptomyces sp. that all treatments resulted in a significant reduction in the percentage and severity of infection in comparison to Fusarium culmorum treatment only, which were (87 and 46%) respectively. The treatment (ST+F.c) with commercial Streptomyces sp. achieved the highest percentage decrease in the percentage and severity of infection (2, 1%) respectively, which was not significantly different from the treatment (N+SB+ST+F.c); this treatment included nanoalgae, local and commercial bacteria compared to the fungi treatment alone, where the percentage and severity of infection for the fungus treatment only was (87%) and (46%) respectively. The study revealed that nano-algae (N) treatments also reduced the percentage and severity of infection with F.c. Furthermore, the results indicated a significant differences in the biochemical indicators to stimulate induce systemic resistance (ISR) in the wheat, and also the plant's nutrient content. The efficiency of the studied treatments proved their effect on the peroxidase enzyme activity, as the treatment (N+A+ SB +ST +F.c) was with high efficacy to elevate the enzyme activity; the enzyme activity was reached (0.74), followed by (A+SB+ST+ F.c) treatment with (0.69). However, each treatment alone achieved an elevation in the activity of peroxidase enzyme. All the studied treatments caused a significant increase in the content of phenols and proteins in the wheat plant compared to Fusarium culmorum treatment; however the treatments were differed in the content of phenols and proteins after 7, 14 and 21 days of treatment. All treatments significantly increased the chlorophyll content in the wheat after 30 days of fungus treatment in comparison to the treatment of pathogenic fungus alone; where the combination treatment (N+A+SB +ST) achieved the highest percentage of chlorophyll content at (18.1), followed by (N+A+SB+ST+Fc) at (16.4) and algae alone (A) at (14.3) which did not differ significantly from nano-algea treatment (N) (13.2).