Thyroid hormone is required for proper development as well as to control blood cell metabolism and proliferation, which affects the metabolism of all cells in the human body. Vitamin B12 is a water-soluble vitamin that deficiency causes a range of ailments. The current study evaluated the levels of vitamin B12, folic acid, and thyroid hormones in hypothyroid people.The study included 384 hypothyroid individuals who visited the OPD of General Medicine at Santosh Medical College. The control group consisted of healthy adults of similar age and sex. The current investigation found that serum vitamin B12 levels were considerably low, whereas TSH, Folic acid, and normal serum T3 and T4 levels were much higher.[1]