Aim: To evaluate the levels of Serum Fasting blood glucose, IR and Serum Ferritin in diagnosed PCOS patients and normal controls. Material and methods: The current study was conducted in urban tertiary hospital based a Casecontrol analytic cross-sectional study in Departments of Biochemistry and Gynecology & Obstetrics of Faculty of Medicine and Health Sciences, SGT University, Budhera, Gurugram, India on 160 subjects aged between 18 to 45 years comprising of 80 healthy control and 80 patients with clinically diagnosed cases of PCOS, acted as study group. The blood samples of controls as well as study groups were withdrawn and Clinical evaluation included anthropometry, Hight, Weight, Body Mass Index (BMI) and waist-to-hip ratio (WHR) were calculated. Circulating levels of fasting glucose, insulin resistance, and Serum Ferritin level were determined by Chemiluminescence based immunoassay (CLIA) on fully automated Biochemistry analyzer MAGLUMI 1000. Results: It is observed that the out of total 160 subjects with both control and study group PCOS patients were included in the study. The age of these control group was ranged from 18 to 45 years, with a mean of 26.23±5.58 years and PCOS study were found 27.76 ±6.54 years. The findings of anthropometric measurements of the subjects of both PCOS syndrome (n=80) and non-PCOS (n=80). As expected, mean levels of BMI, Weight, Height showed marked difference and were statistically significantly increased (P<0.0001) in both the groups. The serum levels of serum Fasting blood glucose level, insulin resistance, and Serum Ferritin were significantly increased (P<0.0001) in subjects with PCOS and without PCOS compared to controls. Conclusion: These results demonstrated that elevated level of serum ferritin was associated with insulin resistance in PCOS women.