Introduction and objective: There is an obvious need to evaluate the knowledge level of dentists about dental stem cells, as a new technology. Hence, the objective of this study was to evaluate the knowledge level of dentists in Kerman city about dental stem cells during the years 2018-2019. Methodology: This descriptive-analytical type of cross-sectional study was conducted on 378 general dentists in Kerman city during the years 2018-2019. Census sampling was used in this study. A researcher-made questionnaire was used to assess knowledge. Pearson correlation, t-test and variance analysis were used to analyze the data. P <0.05 was considered as a significant level. Results: The mean score of dentists' knowledge was obtained 13.16 ± 3.55 in this study. There was no significant relationship between the knowledge level of dentists and basic skill and initial place of starting to work (P = 0.865 and P = 0.974, respectively). However, there was a significant relationship between dentists' level of knowledge and gender, age, history of participation in related seminars, duration of activity (employment history), rate of study of scientific articles and willingness to participate in training courses (P = 0.008, P = 0.008, P =0.0001, P =0.03, P =0.0001 and P =0.0001, respectively). Conclusion: The results of this study showed that dentists' knowledge about dental stem cells is not at the acceptable level and it is possible to enhance the knowledge level and clinical application by providing the necessary resources and trainings